Extending the Detection Limit in Fragment Screening of Proteins Using Reverse Micelle Encapsulation
نویسندگان
چکیده
منابع مشابه
Detection of secondary binding sites in proteins using fragment screening.
Proteins need to be tightly regulated as they control biological processes in most normal cellular functions. The precise mechanisms of regulation are rarely completely understood but can involve binding of endogenous ligands and/or partner proteins at specific locations on a protein that can modulate function. Often, these additional secondary binding sites appear separate to the primary bindi...
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Perhaps 5%-10% of proteins bind to membranes via a covalently attached lipid. Posttranslational attachment of fatty acids such as myristate occurs on a variety of viral and cellular proteins. High-resolution information about the nature of lipidated proteins is remarkably sparse, often because of solubility problems caused by the exposed fatty acids. Reverse micelle encapsulation is used here t...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2019
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.9b00537